Next-generation sequencing (NGS) technology has fundamentally changed genomic research and now is entering the realm of routine diagnostics. Targeted resequencing, or amplicon sequencing, where selected regions of the genome are enriched prior to library preparation by highly multiplexed PCR, allows researchers to focus sequencing power on specific genes or regions of interest, and is often used for the discovery of rare somatic mutations in complex samples or for the analysis of highly complex metagenomics samples.
Speed is critical, especially when examining patient samples. Many clinical facilities are pushing hard to speed up and simplify their sequencing pipeline.
How fast is your amplicon library prep? Traditional library prep can be laborious, taking from 2 to 3 hours. It usually involves sequential enzymatic steps, including end-repair, A-tailing and ligation. If we combine these steps, we can streamline the entire NGS library preparation process and simplify the procedure. Now at QIAGEN, we have accomplished this mission!
With the QIAseq 1-Step Amplicon Library Kit, you can prepare high-quality, artifact-free libraries, ready for use on any Illumina platform in 30 minutes. Using a proprietary end-repair and ligation reaction, the kit generates libraries from PCR products in a single, benchtop reaction! In addition to being faster, the procedure requires minimal hands-on time and fewer pipetting steps, reducing the risk of errors or contamination.
The kit contains high performance reagents for library amplification and has been tested at extremely low amounts of input material, which can be important for larger gene panels and clinical samples. If sufficient starting material is available, such as with 16s targeted microbiome analysis, it can be completely PCR-free to reduce PCR bias and cutting down protocol time even furthe