By learning how a recently discovered immune cell works in the body, researchers hope to one day harness the cells to better treat allergies and infections, according to new Cornell University research.
Type 1 regulatory (Tr1) cells are a type of regulatory immune cell that help suppress immune responses, including inflammation and tissue damage, but very few details were known about their development and function.
A new study with mice and humans, published in the journal Nature Communications, describes how an enzyme called ITK plays a crucial role in the development of Tr1 cells during an immune response. The enzyme offers an entry point for researchers to manipulate the development of Tr1 cells to enhance them to treat allergies, for instance, or block their development to treat viral and bacterial infections.
"The more we understand about how these cells develop, the signals and pathways they use, the more likely we'll be able to devise approaches to manipulate them," said Avery August, professor of microbiology and immunology in Cornell's College of Veterinary Medicine and the paper's senior author. Weishan Huang, assistant research professor of microbiology and immunology, is lead author.
Doctors employ antigen immunotherapy to treat allergies by administering a regimen that exposes a patient to increasing doses of an allergen over a period of months. Since allergies are caused by an overactive immune response to an allergen, the treatment works because Tr1 cells help suppress the immune system and lower inflammation. In the future, clinicians may want to enhance the pathway to produce more Tr1 cells, August said.
But when treating viral infections such as the flu, bacterial infections and tumors, clinicians may want to selectively block the pathway to lower the number of Tr1 cells. In experiments with mice, August and colleagues found that Tr1 cells increase when a mouse is infected with viruses or bacteria or when fighting tumors. By tempering the development of Tr1 cells, and carefully reducing their activity to suppress the immune response, patients may recover faster from certain diseases.
"This is a balance because these cells are there for a purpose, and we think their purpose is to make sure the immune system doesn't destroy and cause pathology in an immune response," August said.
The danger with flu, for example, is that at a certain point other types of immune system T cells, whose purpose is to kill infected cells, start to destroy tissue. In such cases, an overactive immune response can lead to death.
"We'd have to do experiments to find out whether we can tune the function of Tr1 cells," August said, "so we balance the beneficial aspects of the immune response with the damaging aspects of the immune response."
In the study, August, Huang and their colleagues bred genetically altered mice so they carried a gene that makes Tr1 cells glow green when they develop, which allows for easy tracking. They then bred another type of mouse that had fluorescent Tr1 cells and also allowed the researchers to specifically block the enzymatic activity of ITK. Using the same protocol, they created a third type of mouse that lacked ITK.
In both the mice where ITK was inhibited and the mice that lacked ITK, Tr1 cells failed to develop. Using blood cells from anonymous human volunteers, they got the same results.
In a second experiment, the researchers identified a second critical enzyme in the pathway that leads to the development of Tr1 cells. This other enzyme, called IRF4, is a transcription factor that regulates the expression of a number of genes and proved key for controlling whether Tr1 cells developed. The team also confirmed that the same pathway exists in people.
الخلايا التنظيمية (TR1) هي أحد أنواع الخلايا التائية المناعية، والتي يصفها الباحثون بأنها المفتاح الرئيسي لعلاج الأمراض المتعلقة بالمناعة. لكن لابد من فهم آلية عملها ومبدأ تطورها لاستهدافها بشكل صحيح في إنتاج الأدوية لعلاج عدة أمراض مثل الحساسية التي نلاحظ خلالها انخفاض في نشاط خلايا (TR1) مما يؤدي لفرط في الاستجابة الالتهابية، أما في حالة الأورام نلاحظ ارتفاع في نشاطها مما يمنع الخلايا التائية المناعية من مهاجمتها.
في دراسة جديدة تمكن الباحثون من تحديد أنزيم يلعب دوراً رئيسياً في عمل خلايا (TR1). فقد قام الباحثون بحقن خلايا (TR1) بالفلور الأخضر للتوهج عند الفئران وقاموا بتثبيط مسار أنزيم (ITK) فلاحظوا فشل خلايا (TR1) بالتطور ونفس النتيجة لُوحظت عند البشر. هذا أكد للباحثين أن أنزيم (ITK) يلعب دوراً حاسماً في تطور خلايا (TR1) لتساهم في قمع الالتهابات ومنع تلف الأنسجة. لذلك فأنزيم (ITK) قد يكون المفتاح لتطوير أدوية جديدة تعتمد على خلايا (TR1) لعلاج العديد من الأمراض مثل أمراض المناعة الذاتية والسرطانات.
أُجري هذا البحث في : Cornell University ونُشر في مجلة : Nature Communications